| 质粒名称: | pcDNA3/HA-SUMO3 (Sentrin 2) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17361 |
| 存储实验室: | Edward Yeh |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3/HA |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SUMO3,Sentrin2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | HA（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | EcoRI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3/HA-SUMO3 (Sentrin 2) was a gift from Edward Yeh (Addgene plasmid # 17361 ; http://n2t.net/addgene:17361 ; RRID:Addgene_17361) |
| 参考文献部分: | Covalent modification of PML by the sentrin family of ubiquitin-like proteins. Kamitani T, Nguyen HP, Kito K, Fukuda-Kamitani T, Yeh ET. J Biol Chem. 1998 Feb 6. 273(6):3117-20. 10.1074/jbc.273.6.3117 PubMed 9452416 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pcDNA3/HA-SUMO3 (Sentrin 2) 5751 bp ds-DNA circular SYN 09-NOV-2018
DEFINITION Mammalian expression of human SUMO3 with HA tag.
ACCESSION .
VERSION .
KEYWORDS pcDNA3/HA-SUMO3 (Sentrin 2)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5751)
AUTHORS Kamitani T, Nguyen HP, Kito K, Fukuda-Kamitani T, Yeh ET
TITLE Covalent modification of PML by the sentrin family of ubiquitin-like
proteins.
JOURNAL J Biol Chem. 1998 Feb 6. 273(6):3117-20.
PUBMED 9452416
REFERENCE 2 (bases 1 to 5751)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 9, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5751
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 898..924
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
mat_peptide 934..1209
/product="human small ubiquitin-related modifier 3"
/label=SUMO3
promoter complement(1304..1322)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(1305..1322)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(1342..1359)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1348..1572
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1618..2046
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1705..1724)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1915..1936
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2055..2075)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2060..2389
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2240..2375
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2302..2321
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2456..3250
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2510..2529)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3120..3139
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 3424..3545
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3461..3480)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3515..3534
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(3594..3610)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3594..3610)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3607..3629)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3618..3634
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3642..3672)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3687..3708
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3825..3842)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3996..4584)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4076..4095)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4755..5615)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5378..5397
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5616..5720)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatgtac
901 ccatacgatg ttccggatta cgctagcgga tccatgtccg aggagaagcc caaggagggt
961 gtgaagacag agaatgacca catcaacctg aaggtggccg ggcaggacgg ctccgtggtg
1021 cagttcaaga tcaagaggca cacgccgctg agcaagctga tgaaggccta ctgcgagagg
1081 cagggcttgt caatgaggca gatcagattc aggttcgacg ggcagccaat caatgaaact
1141 gacactccag cacagctgga gatggaggac gaggacacca tcgacgtgtt ccagcagcag
1201 acgggaggtg tgccggagag cagcctggca gggcacagtt tctgaattct gcagatatcc
1261 atcacactgg cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa
1321 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
1381 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
1441 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
1501 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
1561 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
1621 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
1681 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
1741 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
1801 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
1861 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
1921 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
1981 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
2041 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
2101 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
2161 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
2221 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
2281 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
2341 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
2401 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
2461 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
2521 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
2581 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
2641 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
2701 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
2761 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
2821 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
2881 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
2941 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
3001 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
3061 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
3121 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
3181 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
3241 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
3301 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
3361 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
3421 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
3481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
3541 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
3601 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
3661 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
3721 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
3781 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
3841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
3901 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
3961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
4021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
4081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
4141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
4201 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
4261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
4321 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
4381 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
4441 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
4501 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
4561 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
4621 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
4681 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
4741 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
4801 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
4861 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
4921 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
4981 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
5041 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
5101 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
5161 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
5221 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
5281 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
5341 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
5401 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
5461 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
5521 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
5581 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
5641 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
5701 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
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